Dr Jacob Grimm and his brother Wilhelm discovered a new enzyme that can catalvse

Question

Dr Jacob Grimm and his brother Wilhelm discovered a new enzyme that can catalvse the conversion of straw into gold ing spinning wheels. This enzyme the brothers have called Rumpelstiltskinase, Rumpelstiltskinase StrawSpinning Wheels O This enzyme is derived by the fractionation of fairy cells. Using a standard curve, the Grimm brothers calculated the protein content of their fractionated fairy cells to be 30 mg/ml The graph below shows the amount of gold produced (in coins/bale) over time 25 15 10 6 inpe (hours) From this data the activity of Rumpelstiltskinase in coins/bale/hour is In later years it was recognised that I con/bale is the equivalent of 1 ?mol/mL From this data, calculate the specific activity of Rumpelstiltskinase

Explanation / Answer

Answer:

Based on the given information:

Protein content of the fractionated fairy cells = 30mg/ml

1) From this data the activity of Rumpelstiltskinase in coins/bale/hour is:

2) In later years it was recognized that 1 coin/bale is the equivalent of 1uMol/mL. From this data, calculate the specific activity of Rumpelstiltskinase

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