4. At left is a portion of microarray analysis, showing only 36 genes. You are l
ID: 277271 • Letter: 4
Question
4. At left is a portion of microarray analysis, showing only 36 genes. You are looking at a cholesterol lowering drug that has been A producing some severe side effecets. You have made cDNAs from a B sample from a normal person (labeled with green fluorescence) and also from a person at your hospital who is in the hospital because of E severe side effects from the drug that they have been talking for a F few months (labeled with red fluorescence). Red and green fluorescing together appears yellow. 12 3 456 a) What exactly from the patients is labeled? (what exactly are you looking at here?) b) What spots (in your opinion) show the most change between the two people, and what is the change for each? e) What additional things/samples/controls need to be looked at for this to have any meaning?Explanation / Answer
Microarray:
Microarray is the recent technique which can be used to study the gene expression of a cell or tissue types. Ten thousands of genes can be studies in a single experiment; it is also called as global expression profiling.
Comparison of normal person gene expression with diseased person gene expression helps to identify the exact gene that cause the disease.
It is more useful in cancer studies. Microarray helps to compare the gene expression levels of normal and cancer patients.
Microarray principle:
The principle of microarray experiment is the complementary base pairing (hybridization) that takes place between the DNA strands.
Steps in microarray:
Results of microarray:
Application of microarray:
Microarray has wide applications in various fields.
Questions:
a. What exactly from the patients is labelled? (what are you looking at here?)
Here we are comparing the gene expression of patient treated with drug for lowering the cholesterol level with normal person’s gene expression.
The patients sample is labelled with Cy5 – which gives red colour.
b. What spots show the most change between the two people, and what is the change for each?
The spot 1A and 4D shows strong fluorescence in the patient.’
The spot 1B shows the strong fluorescence in the normal person.
Changes between two people:
The genes only expressed in patient: 1A, 4C and 4D.
The genes only expressed in normal person: 1B, 5B, 5D, 6A.
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