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Yested in studying protein Y because it binds to your favorite protein a. What m

ID: 277246 • Letter: Y

Question

Yested in studying protein Y because it binds to your favorite protein a. What modification was s to X after X has been covalently modifled by a kinase. made to protein X by the kinase? b. Id entify possible amino acids in Y that would be capable of interacting with the modified group (answer you gave in 4a) on protein X. 5. A section of DNA isolated from a rare frog species reads: 5' GCATCCAGCAATACGT 3 Using the genetic code table below answer the following questions Stop codon Stop codon Leuaine rginine AGU Sarne AUA Arginine GUU Gyne A GUA Valine GCC A. Suppose that mRNA is transcribed from this section of DNA using the complementary strand of this segment of DNA as a template. What will be the sequence of the mRNA in this region (make sure you label the 5' and 3'ends of the mRNA and use 1 letter abbreviations)? B. Translate this mRNA into the 3 possible reading frames using the 1 letter abbreviations. If reading frame 2 is the correct reading frame, write out the amino acid sequence using the 1 letter abbreviations. C.

Explanation / Answer

1a. Protein kinases phosphorylates the protein and adds an phosphate group to amino acids.

1b. Most commonly phosphorylated aminoacids are Tyrosine ams Serine. It changes polarity and confirmation of aminoacids and bounds with hydrophillic aminoacids.

2a. mRNA would be-

5'GCAUCCAGCAAUACGU3'

2b. Three possible reading frames are

1. 5' GCA UCC AGC AAU ACG 3'

One letter- ASSDI

2. 5' CAU CCA GCA AUA CGU 3'

One letter- HPAIR

3. 5' AUC CAG CAA UAC 3'

One letter- ILEY

2c. Amino acid seq. - HPAIR