1412/162.101 MAN Distance ?? ASR NSB SC QUESTION 10 a) Most of the enzymes and o

Question

1412/162.101 MAN Distance ?? ASR NSB SC QUESTION 10 a) Most of the enzymes and other components used in DNA technology (for example, to clone genes in E. col) were originally derived from living cells, where they play important roles. Fill in the blank fields in the following table to describe their specific roles in these two situations. [8 marks] Enzyme/ component Description of usual Description of role in biological role in cell D DNA technology Primer DNA polymerase Restriction enzyme DNA ligase

Explanation / Answer

1) Primer - used in DNA replication process in cell

used to bind and help in amplifying specific DNA in rDNA technology.

2) DNA polymerase - used in DNA replication.

used to amplify specific DNA .

3) Restriction Enzymes- used to cut the specific site on DNA.

help in creating new chimeric DNA products.

4) DNA ligase - joins Okazaki fragments after replication.

join different type of DNA to make chimeric DNA products.

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