iples of Gel Fiftration Chromatograh udy Questions Answer the following study qu

Question

iples of Gel Fiftration Chromatograh udy Questions Answer the following study questions in your laboratory nctebock or on a veon n worksheet. 1. Compare gel filtration chromatography to other ted hniques of separ ation Draw and label the parts of a column. Include the amounts of butter which 'k olaced above/below the bed. Define: frit, matrix, and eluant 3. What is the purpose of an elution buffer. 4. Which would elute from a column first: a spherical or a linear molecule? hy 5. What is the stationary phase of the chromatography? The mobile phase

Explanation / Answer

gel filtration chromatography is based on the size of the protein. more the size faster is elution and smaller in size slower in elusion from the column.

it is different from hydrophobic interaction chromatography because it does not require treatment with high salt to expose hydrophobic residues of the protein to interact with resin.

also it different from ion exchange which involves ionic interaction between protein and resin for separation.

it is different from SDS PAGE because it does not require SDS treatment to linearize/denature the protein.

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