You are studying apoptosis in a particular cell type and you would like to deter

Question

You are studying apoptosis in a particular cell type and you would like to determine if caspase activation occurs during this apoptotic event. b. (4pts) Describe how are caspases normally activated during apoptosis (4pts) Design an experiment to test if caspases are active during your apoptotic event. This is just the experimental design...what are you going to do (not what will it show you) c. (6 pts)-Draw and label two figures with all of the appropriate controls. You do not need to write a figure legend, but your labeling should be sufficient to allow me to interpret your figure. d. i. ii. A figure showing that caspases ARE activated by your apoptotic event A figure showing that caspases ARE NOT activated by your apoptotic event

Explanation / Answer

(b),

Most of the caspases play an important role in the apoptosis, which is described as programmed cell death.

The activation of the caspases involves the stages namely, dimerization and cleavage.

The dimerization process is facilitated by the binding to the adaptor proteins.

This binding is through the protein-protein interaction. Some of the activating multiprotein complexes formed during the caspase activation are:

Inflammasome

Apoptosome

Death-inducing signaling complex.

In the cleavage stage, at the inter domain linker regions, the caspases cleave. This leads to the formation of small and large subunits. It helps the active-site loops to take the information which is favorable for the enzymatic activity.

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