1701/162.101 MANIALB Internal only ?? ASR TR NSB Sc QUESTION 12 a) An organic fa

Question

1701/162.101 MANIALB Internal only ?? ASR TR NSB Sc QUESTION 12 a) An organic farmer states that none of his crops are student designs a PCR experiment to amplify a 900 bp frag nts ne, which is only found in genetically modified plants. He samples endotoxin gen genetically modified. A plants and produces the PCR gel 1 shown below. The labeling indicates a ladder, positive and negative controls, and ten lanes of samples. DNA size Gel 1 Gel 2 Samples Samples 1 2 3 4567 8 910 11 12 13 1 2 3 4 5 6 7 8 9 10 11 12 13 0 Based on Gel 1, are any of the ten plants genetically modified? If so, indicate which lanes have samples from genetically modified plants by circling the sample numbers above the lanes in gel 1. Explain your answer. (4 marks) (i) The farmer complains about this result. Looking back at his lab notebook, the student realized that he mislabeled the lanes. The correct labeling is shown in Gel 2. Based on Gel 2, are any of the ten plants genetically modified? Explain your answer. (2 marks)

Explanation / Answer

a 900 bp gene amplification will tell you that your plant is genetically modified.

in gel 1. plant sample lane - 2,3,5,7,8,9,10, and 11 are GM samples.

now the gel 2 contains correct labeling than plant sample lane - 5,7,8,9,10,11 and 13 are GM plants.

though in gel 2 the negative control also has 900 bp amplified product.

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