6. Given the following template strand of DNA, answer the following questions: 2

Question

6. Given the following template strand of DNA, answer the following questions: 20 ps.) (a) Write the coding (complementary) DNA strand (b) Write the mRNA that would be transcribed from the template strand (the one provided). (c) Write the tRNA anticodons that match the mRNA codons. (d) Using the genetic code table below, write the amino acids (in sequence) that would actually form the resulting polypeptide chain. [Just use the 3 letter abbreviations found in the table.] For e-h what effect would the mutation have on the amino acid sequence and what effect would you expect the mutation to have on protein formation and function and why. Start with the original sequence for each question (i.e. do not carry the mutations through to the next question) (e) If the last nucleotide of the DNA strand was mutated from a C to a T what effect would you expect it to have on protein formation and why? (0) If the 9th nucleotide of the DNA strand was mutated from an A to a G what effect would expect it to have on protein formation and why? you (g) If an A G were inserted between the 7h and 8th nucleotides (becoming GAGT) what effect would you expect it to have on protein formation and why? (h) If the 6th nucleotide of the DNA strand was mutated from a C to a G what effect would you expect it to have on protein formation and why?

Explanation / Answer

Template strand = 3'-GCATACGTAGCTGAGTCCCGAACTC-5'

(a) Coding strand = 5'-CGTATGCATCGACTCAGGGCTTGAG-3'

(b) mRNA sequence = 5'-CGUAUGCAUCGACUCAGGGCUUGAG-3'

(c) tRNA anticodons = 3'-GCAUACGUAGCUGAGUCCCGAACUC-5'

(d) amino acid sequence = N-Arg-Met-His-Arg-Leu-Arg-Ala-C

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