Question 1. Which of the following statements about bacterial transcription are

Question

Question 1.

Which of the following statements about bacterial transcription are true?

a. sigma factor interacts with the -10 box in bacterial promoters to increase transcription

b. bacteria often have several genes that encode sigma factors with different specificity

c. intrinsic terminators are stem loop structures that interact with the Rho protein

d. RNA polymerase melts the DNA to form a transcription bubble to access the template strand

e. Transcriptional initiation starts when the RNA polymerase interacts with a Shine-Dalgarno sequence

f. TATA binding protein plays a key role in binding to the TATA box of bacterial promoters

Question 2. When phage infect a bacterial cell, they typically have a temporal program of gene expression, i.e., they express particular genes at particular times. What are two regulatory mechanisms phage use to control their gene expression?

Question 3. Transcriptomics and proteomics are two global approaches for studying cellular responses. Briefly describe how these approaches differ.

Explanation / Answer

a) Yes its true it interacts with 10 box in bacterial promoters to increase transcription it has to enable binding of RNA polymerase to gene promoters to initiate transcription.

c) The intrinsic terminators interact with the Rho protein because it stops and releases the newly made RNA . This is the mechanism of intrinsic termination.

d) Yes its true RNA polymerase melts the DNA to form a transcription which occurs through the change in biomolecules.

2) When the bacterial cell is infected by a phage they have a temporal program of gene expression and they can express particular genes at particular times.

And the regulatory mechanisms are used to control the gene expression they are lysogenic life cycpe and lytic life cycle; these integrates into the host after the phage initially infects the bacterial cell.

3) Transcriptomics is the study of transcriptomes which are known as the set of RNA molecules among cells.

For the transcriptomatic profiling some techniques are used and DNA microarray is the mostly used technique and its an inexpensive analog technique.

Proteomics: Proteomics is the study of proteomes where the proteomes are known as the set of proteins produced in an organism.

It is the study which helps to understand the structure; function and interaction of the protein.

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