A researcher performed complementation analysis on a set of mutants he isolated

Question

A researcher performed complementation analysis on a set of mutants he isolated for a biosynthetic pathway and found 5 complementation groups (i.e, 5 genes were involved in the pathway). He named them 1, 2, 3, 4 and 5 for the time being. He also knew that there were 6 metabolites in the pathway, A, B, C, D, E and F. The table below shows you his results of growing the mutant strains on minimal media alone, and when supplemented with the various metabolites (+= growth, - = no growth):

Minimal Media supplemented with:

a) What is the order of the metabolites in the pathway?

b) What is the order of genes in the pathway?

Genes: Nothing +A +B +C +D +E +F 1 - - - + - + - 2 - + + + - + + 3 - - + + - + + 4 - - - + - - - 5 - - - + - + +

Explanation / Answer

a) order of the metabolites in the pathway:

D --> A --> B --> F --> E --> C

b) order of genes in the pathway:

4 --> 1 --> 5 --> 3 --> 2

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