Q1: Do you think the optimum pH for this enzyme is consistent with the fact that

Question

Q1:  Do you think the optimum pH for this enzyme is consistent with the fact that the bacterium that produces it is an acidophile?

Q2: What is the ‘substrate’ for an amylase enzyme, and what is the ‘product’ of the reaction?

Q3: Why do you think the enzyme efficiency, as measured in this experiment’ never reaches 100%?

Q4: Notice that the pH and temperature data form two ‘peaks,’ while the substrate data forms a ‘plateau.’ The plateau is reached when you give the enzyme the optimum amount of substrate to work with, but neither increases nor decreases when you give it any more substrate. Can you explain why this would be the case?

Table 1: Amylase Enzyme Efficiency as a Function of pH, Temperature, and Substrate Concentration Enzyme Efficiency Temp. Enzyme (C) Efficiency Substrate Concentration Efficiency (mM) pH Enzyme 20 28 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 0 10 15 20 25 30 35 40 45 50 10 15 20 25 30 35 40 45 50 12 31 40 31 12 36 38 40 41 42 42 42 42 42 42 42 15 25 60 65 70 60 65 2 70

Explanation / Answer

1) the optimum pH range of this enzyme is near to pH 1.5-2.5. the producer is an acidophile.

2) substrate for amylase is starch. it converts linear starch (amylose) into maltose units.

3) the experiment is not conducted at optimum pH, temperature, and saturated substrate concentration. so it will never reach 100%.

4) saturated substrate concentration means the amount of substrate is sufficient to occupy all the available active sites of the enzyme present in the reaction. it is necessary to determine the activity of the enzyme otherwise the free enzyme will show the increase the reaction rate in an experiment.

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