ment 7- Due x /leca ssessment/take/launch.jsp?courseassessment.jd 165242 1&cours

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ment 7- Due x /leca ssessment/take/launch.jsp?courseassessment.jd 165242 1&course; id-101890 1&conter; Question Completion Status: QUESTION 12 1 points The next 3 questions are challenge questions, You should expect that they will take you some time and may benefit from working in a group with your peers You have completed the sequencing of many parts of the dystrophin gene. Which of the following pieces can NOT be included in the final contig? In order to assemble into a contig, there should be at least 6 bases of overlap at the end of the strand. (Consider why it's not enough to have overlap in the middle of the strand). Note that some sequences were sequenced on the forward strand and others on the reverse strand 1. 2. GAAAAAGTGT750bp TGTAAA O Piece 1 O Piece 3 O Piece 5 O Piece 6 QUESTION 13 1 points You wish to map the position of anpelin resistance onthe ntoctious pasmid?You mx a cel that is ampicilin resistant and contains genes X and Y with a strain that is amoicilin sensitive and lacks genes X Cluck Sqve and Submit to sqve and submit. Click Save All Answers to sqve all answers Save and MacBook Air ?? F4 FS F6 F7 69

Explanation / Answer

Answer: Option 5 is correct.

Explanation:

i. 3'-end of 1 and 5'-end of 2 have identical sequences (GAAAAA). So, they are adjacent contigs.

ii. 3'-end of 3 and 3'-end of 4 have complementary sequences (TTTACA/AAATGT). So, they are adjacent contigs.

iii. 3'-end of 6 and 5'-end of 3 have identical sequences (AGTTT). So, they are adjacent contigs.

Contig 5 has no identical or complementary sequences at its ends with other contigs.

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