Using a droplet based digital PCR system a DNA sample in a 1?L tube will be test

Question

Using a droplet based digital PCR system a DNA sample in a 1?L tube will be tested in droplets with a diameter of 20?m.
A) How many droplets are needed to test this sample?
B) What should be the minimum initial concentration so that there is less than 0.01% chance of finding two molecules in each droplet? Using a droplet based digital PCR system a DNA sample in a 1?L tube will be tested in droplets with a diameter of 20?m.
A) How many droplets are needed to test this sample?
B) What should be the minimum initial concentration so that there is less than 0.01% chance of finding two molecules in each droplet?
A) How many droplets are needed to test this sample?
B) What should be the minimum initial concentration so that there is less than 0.01% chance of finding two molecules in each droplet?

Explanation / Answer

Volume of droplet = 4188µm3

The droplet copy number concentration, Td, was calculated by multiplying M by 1000/Vd

here it is given that there is one molecule per droplet

so 1 = M*1000/v

1= M*1000/4188

M = 4.188 µm3

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