20) By comparing the G+C content of organisms, we can determine if they are not
ID: 274443 • Letter: 2
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20) By comparing the G+C content of organisms, we can determine if they are not related. This approach is ultimately based upon A) the idea that two unrelated organisms can never have the exact same number of G-C pairs B) counting up the exact number of G-C pairs. C) None of the choices are correct. D) us sequencing out the genome to discover all the locations where there are G-C pairs. E) the fact that G-C pairs form 3 hydrogen bonds, whereas A-T pairs form 2 hydrogen bonds, meaning that sequences rich in G-C pairs will require a higher temperature to separate the double stranded DNA into single strands. 21) After digesting the genomes of two unknown bacteria with a restriction enzyme, running the resulting fragments out on a gel, and comparing the resulting banding patterns of the two lanes, we find that these two patterns are different. What does this tell us? A) The two bacteria are identical. B) The two bacteria are closely related. C) We can't tell anything from this result. D) This is called an RFLP, but this technique is actually meant for a different purpose. E) These two bacteria are different.Explanation / Answer
20) Evaluating whether organisms are related by determining their G+C content is based upon
D) us sequencing the genome to discover all the locations where there are G-C pairs.
Sequencing and analytzing trends between various genes, conserved motifs across same genes, can determine if organisms are related to each other.
21) Since the pattern for two bacteria is different, option E) is correct. The bacteria are different.Genotypically different, and RFLP or Restriction fragment length polymerism technique used is based upon different position of restriction sites. These bacteria have different positions of restriction sites, often created by mutations, hence a different pattern, These bacteria could be however, related or of same species.
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