To observe luciferase activity in Arabidopsis plants expressing the luciferase g

Question

To observe luciferase activity in Arabidopsis plants expressing the luciferase gene, you must first spray them with a chemical called luciferin. Which of the below statements is not correct? (choose one )

Luciferase is a substrate for luciferin.

Plant cells can take up luciferin from an aqueous solution.

c.Fireflies make luciferin but plants normally do not.

d.Luciferin is required for light production by luciferase.

Luciferase is a substrate for luciferin.

Plant cells can take up luciferin from an aqueous solution.

Explanation / Answer

The correct option is Option A “Luciferase is a substrate for luciferin”. In fact, it is the other way around.

Luciferin is a protein that is generally regarded as the molecule responsible for bioluminescence. It is a light-emitting molecule found in certain organisms. In order to produce light, these proteins undergo enzyme-catalysed oxidation , mediated by the enzyme Luciferase. Therefore, it can be said that luciferin is the substrate for luciferase. The molecule, upon oxidation, undergoes a transition to an excited state, and emits light upon decaying to its ground state.

Luciferin and luciferase can be packaged into nanoparticles and suspended in solution. These can be taken up by plants through stomata, and cause the leaves to glow.

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