XPERIMENT 5: ENZYME PURIFICATION AND ASSAY IMPORTANT QUESTIONS TO CONSIDER Caref
ID: 273373 • Letter: X
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XPERIMENT 5: ENZYME PURIFICATION AND ASSAY IMPORTANT QUESTIONS TO CONSIDER Careful consideration of the following questions related to this set of experiments will not only insure that you understand the most important concepts of this laboratory but will also help you prepare for laboratory quizzes and the lecture portion of the course. By addressing these questions (and others that you decide are important) in the conclusions section of your laboratory report you will also assure that you receive full credit for that portion of your report HOW DID THE total amount of protein change as you proceeded through each step of this experiment? What fraction of the original amount of protein was present at the last step relative to when you began? HOW DID THE total amount of wheat germ acid phosphatase activity change as you proceeded through each step of this experiment? What fraction of the original amount of acid phosphatase activity was present at the last step relative to whern you began? HOW MUCH DID you purify wheat germ acid phosphatase? (Divide the amount of enzyme activity per mg of protein at the end of the experiment by the amount of enzyme activity per mg of protein at the start of the experiment.) WHAT STEPS COULD you take to further purity wheat germ acid phosphatase? 5 WHY WAS IT important for you to keep your samples cold until the enzyme activity assay steps? ACID PHOSPHATASES ARE produced by many human tissues such as the liver, kidney, spleen and prostate gland. The prostate gland enzyme, but not that of other tissues, is inhibited by tartrate ions. Prostate gland acid phosphatase levels in blood (determined with the same assay that you used) can be an important indicator of prostate cancer. How could this information be used to develop a human blood test for prostate cancer? G.Explanation / Answer
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