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Most colonies on streak plates grow from isolated colony-for a colory can be a m

ID: 272950 • Letter: M

Question

Most colonies on streak plates grow from isolated colony-for a colory can be a micture of two different organisms. If a culture is sta correct identification will be next to impossible because the extra organism could con results. How could you verify the purity of a colony? (Th you have bad prior to performing this exercise.) If you found the colory to be a m rming units (CFUs). On rare occasions, however rted from this colony (thinking it is pure), found the identifying test e answers may vary depending on what experience d prior to performing this exercise.) If you found the colony to be a mixture of organisms, what could you do to purify it?

Explanation / Answer

The best method to purify a mixture of colonies is to dilute it. Statistically speaking, as you increase the amount of solution the colonies, will have larger volume to occupy. Now this diluted solution can be plated. As you have reduced the concentration, chances are that you will get distinct colonies. Dilution can be carried out by following

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