What is the functi f Dt . What is the function of RNA polymerase? 12. What is th

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What is the functi f Dt . What is the function of RNA polymerase? 12. What is the function of reverse transcriptase? 13. What is the function of PAP? 14. A typical polycistronic mRNA MAY contain all of the following EXCEPT (A) a leader region (B) start codons (D) a ribosome binding site (E) a 5 cap (C) stop codons 1S. Lagging strand synthesis involves kazaki fragments (B) Shine-Dalgarno fragments (C) Klenow fragments (D) restriction fragments 16. When a messenger RNA molecule arrives at the ribosome O ít binds to the 16SRNA ?n the small subunit (E) ?t binds to the 5S RNA in the large subunit (A) it binds to the A-site (B) it birds to the P site (D) it binds to the 23S RNA in the large subunit 17. In prokaryotes, rho-independent terminators are characterized by RNAs that have an struct ure followed bys ) inverted repeat, poly(U) tract (C) 8-oxoguanosine diphosp RNA poly (A) inverted repeat, poly(T) tract (B) inverted repeat, poly(G) tract (E) direct repeat, poly(A) tract (D) direct repeat, poly(C) tract 8. The 5-cap on a processed mRNA consists of (B) several adenosine nucleotides (E (A) N-formyl methionine (D) 7-methyl-guanosine triphosphate 5-methyl-adenosine-triphosphate 19. The E. coli RNA polymerase is involved in transcription initiation; whereas the involved in elongation. (B) core enzyme; holoenzyme (D) core enzyme; core enzyme (A) holoenzyme; core enzyme (C) holoenzyme; holoenzyme uences of DNA that can move around to 20. In 1 940, Nobel Laureate Barbara McClintock discovered seq positions within th e maize genome and cause mutations. These sequences are (A) transposons (B) inverted repeats (C) direct repeats (D) introns (E) exons me (made of proteins and RNA) that elongates chromosomes by adding TTAGGG sequer end of existing chromosomes is (B) exonuclease (C) telomerase (D) amylase (E (A) endonuclease 22. In the formation of an aminoacyl tRNA (A) the amino terminus of the amino acid is directly attached to the 5' end of the tRNA (B) the carboxyl terminus of the amino acid is directly attached to the 5' end of the tRNA the amino terminus of the amino acid is directly attached to the 3' end of the tRNA (D) the carboxyl terminus of the amino acid is directly attached to the 3' end of the tR (E) the side chain group of the amino acid is directly attached to the anticodon loop of the tRNA 23. An E coli cell that has the genotype l'OZ:/TO'Z' would (A) repress ß-galactosidase synthesis upon the addition of lactose (B) constitutively synthesize B-galactosidase (C) be unable to synthesize p-galactosidase (D) only induce ?-galactosidase synthesis upon the addition of lactose (E) none of the above 24. An allosteric protein is (A) A protein that binds to DNA 1 nrotein that changes shape when it binds a small molecule

Explanation / Answer

14) Answer is E

In eukaryotes, the 5prime cap (cap-0), found on the 5prime end of an mRNA molecule, consists of a guanine nucleotide connected to mRNA.The 7-methylguanosine cap is essential for mRNA translation and cell viability from yeast to mammals.

18) Answer D

19) Answer is A:  

E. coli RNA Polymerase Core Enzyme consists of 5 subunits designated alpha, beta, beta prime, omega and sigma. core enzyme is free of sigma factor and does not initiate specific transcription from bacterial and phage DNA promoters whereas holoenzyme consists of sigma factor and involved in transcription initiation

20) Answer A: transposans

21) Answer C: telomerase

11) RNA polymerase is an enzyme that is responsible for copying a DNA sequence into an RNA sequence, during the process of transcription.

An enzyme that binds to DNA during transcription and separates or unwinds the DNA strands then uses one of the strands as a template from which to assemble nucleotides into a complementary RNA strand

12) A reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed as reverse transcription. It is mainly associated with retroviruses.

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