3) (34 pts) In a newly discovered animal, chromosome 3 contains a dominant mutat

Question

3) (34 pts) In a newly discovered animal, chromosome 3 contains a dominant mutation stripe, which causes a recessive lethal phenotype during larval stages. Several mutant animals carrying recessive lethal mutations on chromosome 3 were isolated and kept in stocks as trans-heterozygotes with stripe (i.e., mutant/stripe). These stocks were then crossed to each other (i.e., mut /stripe x mut2/stripe and their progeny (including dead carcasses) were examined. Listed in the table below is the number of progeny that are wild type (i.e., NOT stripe). 25010- 221 250 0 300 0 360 0 280 120 140 0 150 180 120 130 180 200 160 225 130 210 158 0 100 175 201 101 130 150 170 0 350 280 260 330 256 0 180 280 0 350 165 190 220 145 3100 250 195 0 A. What are the complementation groups? B. You isolate 9 additional non-lethal mutants that are in the same complementation group as mutant 3. primer 2 The sequence below is the region surrounding gene X. Design DNA primers 1 and 2 (5 nucleotides ONL Y) to amplify this region. Indicate the polarity of the primers. A AGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGc I. Primer 1: 5' II. Which is the template strand for transcription? Circle ONE: A III. Which is the 3' end of the DNA? Circle ONE: A or B Primer 2: 5'- or B

Explanation / Answer

6 Complementation groups

complementation groups-- (1,5), (2,4), (3), (6,9), (7,10), (8)

B.

Strand A- 5’-3’

Strand B- 3’-5’

I. Primer1: 5’-AGCAT-3’

Primer2: 5’-CTCGC-3’

II. Template strand for transcription- strand B

III. 3’ end- B

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