WHICH OF THE FOLLOWING IS FALSE? A. If lox-P sites are direct repeats (repeats f

Question

WHICH OF THE FOLLOWING IS FALSE? A. If lox-P sites are direct repeats (repeats face same direction), CRE B. If lox-P sites are inverted repeats (repeats face opposite C. If the promoter/enhancer for the CRE-gene is liver-specific, D. This "knock-out" strategy is useful for genes that are essential to induces deletion of the gene directions), CRE induces deletion of the gene deletion of the gene will occur only in the liver development but are also expressed in particular tissue types in adult animals

Explanation / Answer

Answer: (B) If lox-P sites are inverted repeats (repeats face opposite directions), Cre induces deletion of the gene

Explanation:

If loxP sites have direct repeats (repeats in same orientation), Cre induces deletion of the gene.

If loxP sites have inverted repeats (repeats in opposite orientation), the gene sequence is inverted.

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