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Need help 14-18 please please 14. Which of these events is not an activity of DN

ID: 272143 • Letter: N

Question

Need help 14-18 please please

14. Which of these events is not an activity of DNA polymerase during DNA replication? a. Continuous 5' to 3' DNA synthesis of the leading strand b. Discontinuous 5' to 3' DNA synthesis of the lagging strands C. 3, to 5, proofreading and 5, to 3. correction of errors d. 5" to 3' synthesis of RNA primer 15. The energy that is needed during the synthesis of DNA is provided by a. The unwinding of the parental DNA molecule. of the terminal phosphate from the incoming nucleotide te. 2 C. DNA polymerase d. Hydrolysis of the energy-rich bond of the P-P removed from the incoming nucleotide triphosphate 16. Differntiate and discuss 5-3' exonuclease, 3-5' exonuclease and endonuclease activity with examples 17 Discuss the differences between prokaryotes and eukaryotes with regard to their DNA replication? What phase of the cell cycle DNA replication happens in prokaryotes? 18. A student wrote a detailed protocol for PCR including components required for PCR He wrote that he used Buffer, Mg++, ddNTPs, Forward Primer, reverse primer, template, Taq polymerase used. If the template is 750 bp long, what is the product size he gets if he performs the experiment. Discuss your interpretation. and water in the reaction. Discuss each one of them why he

Explanation / Answer

Q.14 answer is d - 5' to 3' synthesis of RNA primer.

Because during DNA synthesis primase enzyme perform function of synthesizing RNA primer.

So it is not function of DNA polymerase.

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