(6) TheA antage of CRISPRCasbased prone editing bol ?s: Cas9 uses a specific DNA

Question

(6) TheA antage of CRISPRCasbased prone editing bol ?s: Cas9 uses a specific DNA in the cell as a guide in recognizing targe gene sece Cas9 can degrade RNA and protein C) Cas9-modiated DNA breakage occurs foe both chromosomes and thus it creaes homorygotes quickly D) Cas?does not have any off-target concern (7) The bess reasonis) foe the use of model organisms in the hiomedical stuadies are that these A) have small genome size. B) have shont life cycles C) can be genetically transformed D) contain genes that have evolutionary conserved functions is humans or crops A, B and C CEAll of the above (8) Which of the following model organisms has the largest genome? A) Arabidopsls Yeast E coli (9) Flower organ pattern formation is specified by the so-called A. B and C genes in Arabidpi. A gene alone makes sepal, A and B together make petal, B and C together make stamen, and C alone makes carpel. If you want to generate a novel plant which has all four whorls of petals (ie without sepals, stamens and carpels), which of the following is the best strategy? A) Inactivate gene C B) Activate genes A and B in all whorls C) In activate gene C and activase gene B in all whorls D) Activate gene B in all whorls. E) None of the above. (10) The difference between transient and stable transformation is A) Transien transformation means the transformed DNA cannot he stably inherited B) Stable transformation means the transformed DNA has been integrated into the host genome C) A and B D) Neither A noe B (11) In generating knockout mice, what is the purpose of using the TK gene as one of the markers? A) Making sare that the selected ES cell contains the antibiotic resistance marker. B) Making sure that the endogenous gene of interest has been replaced by the knockout version of the target gene C) Both A and B D) None of the above

Explanation / Answer

6. Ans- C. cas9- mediate DNA breakage occur for both chromosome and thus it creates homozygotes quickly
Explanation:
The CRISPR-Cas9 genome editing system has two components such as a “guide” RNA (gRNA) and a non-specific CRISPR-associated endonuclease (Cas9). The CRISPR-Cas9 system is able to modify the target DNA with high fidelity than existing technologies such as TALEN and ZFN.
The main advantages of CRISPR/Cas9 system are its simplicity and efficiency. The endonuclease Cas9 protein uses gRNA to form base pairs with DNA target sequences and form a site-specific double-stranded break in the DNA.
7. Ans-F. all of the above
Example:
The unique characteristics of a model organism that are used in biomedical studies include:
They have short life-cycle, easily manipulated, a short lifespan, produce a large number of offspring and have a sequenced genome. Sponges are an important model organism that used for studying ancestral metazoan homologs and also used for studying the basic physiological function of cancer genes in simpler animals.
8. Ans- C. Mouse
Explanation:
The genome size of the followings include:
Arabidopsis- 100 million
Drosophila- 120 Million bp
Mouse- 3 billion
Yeast- 15 million bp
E. Coli- 4.6 million bp

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