The DNA below contains part of a prokaryotic promoter and gene. The promoter is

Question

The DNA below contains part of a prokaryotic promoter and gene. The promoter is in bold and the transcription start site is underlined.

5' TAGAACGATTTAATATTGATGATAAGTCGTTAGACGCGTAAAA 3'

3' ATCTTGCTAAATTATAACTACTATTCAGCAATCTGCGCATAAAA 5'

What is the sequence of the mRNA that would be transcribed? For full credit, label the 5’ and 3’ ends of the strand.

Write out the sequence of the polypeptide that would be made from this gene. For full credit, label the C- and N-termini.You may use the codon table from your book or look one up.

Explanation / Answer

Answer:

DNA informational strand: 5'TAGAACGATTTAATATTGATGATAAGTCGTTAGACGCGTAAAA3'

DNA template:5’ TAG AAC GAT TTA ATA TTG ATG ATA AGT CGT TAG ACG CGT AAA3’

mRNA codon: 5’ AUC UUG CUA AAU UAU AAC UAC UAU UCA GCA AUC UGC GCA UUU 3’

Hence,

Polypeptide: ILE LEU LEU ASN TYR ASN TYR TYR SER ALA ILE CYS ALA PHE

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