4. You and your postdoctoral mentor are testing two newly developed anti-cancer

Question

4. You and your postdoctoral mentor are testing two newly developed anti-cancer drugs. You have obtained two sets of patient samples to test the drugs on: cancer cells and normal cells. You go into lab to finish an experiment for your postdoc while they are at a conference. Your postdoc's handwriting is terrible, so you aren't sure which plate has the cancer cells and which has the normal cells based on their labeling. You do know that one plate was seeded with cancer cells, and one plate was seeded with normal cells. You also know that you and the postdoc included 2 wells treated with NO drug, 2 wells treated with Drug A, and two wells treated with Drug B. You decide to finish the experiment anyway and check in with your postdoc when they return. You stain the cells with crystal violet to identify the level of foci formation on all plates. Below are photos of the results you obtained. Cell samples: 1)Pancreatic cancer cells from a patient tumor OR 2)Normal cells from normal patient tissue Treatments: No drug, Drug A or Drug B (All treatments were completed in duplicate.) Results: PLATE 2 PLATE 1 2 3 2 2 2

Explanation / Answer

D.So proteins target could be - Bcl-2 or Bcl-XL

E. overexpressed gene in NIH 3T3 cells. When NIH 3T3 cells divide normally in culture, they do so until they reach a confluent monolayer. However, in the presence of an overexpressed oncogene, these cells can begin to grow in dense, multilayered foci1 that can be visualized and quantified by crystal violet or Hema 3 staining.

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