a) In DNA microarrays, the typical probe is~20 nucleotides. Describe the effects
ID: 271713 • Letter: A
Question
a) In DNA microarrays, the typical probe is~20 nucleotides. Describe the effects of using a shorter probe on the sensitivity and selectivity of an analysis. (1 sentence each) Sensitivity: Selectivity b) Describe the effects of using a longer probe on the sensitivity and selectivity of an analysis. (1 sentence each) Sensitivity: Selectivity: c) Would a shorter or longer probe be more effective at distinguishing betweena target DNA and a single-nucleotide polymorphism of that DNA? Circle one: shorter probe longer probe Explain (1 or 2 sentences): d) Describe some of the similarities and differences between sequencing and ne technique v Similarities (2 sentences): Differences (2 sentences):Explanation / Answer
a) Shorter Probe
Sensitivity: Shorter probes have less sensitivity as the strength of the hybridization signal is poor
Selectivity: Greater selectivity as the shorter probes have less tolerance to mismatches.
b) Longer Probe
Sensitivity: Strength of the hybridization signal and sensitivity increases with increase in length of probe. So more sensitivity for longer probe.
Selectivity: Longer probes have higher melting temperatures and greater mismatch tolerance leading to decreased specificity.
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