assignment You are given two solution containing different purified DNAs. One is

Question

assignment

You are given two solution containing different purified DNAs. One is from a bacterium and
has a G+C composition of 68%, whereas the other is from a mammal and has a G+C
composition of 42%.
(a) You measure the absorbance of UV light of each solution as a function of increasing
temperature. Draw the resulting melting curves (light absorption as a function of
temperature.
(b) Which solution will yield the higher Tm value and why?
(c) After melting the two solutions, mixing them together, and allowing them to cool, what
would you expect to happen?
(d) Would appreciable amounts of bacterial DNA be found associated in a helix with
mammalian DNA? Explain.

Explanation / Answer

After melting the two solutions, mixing them together, and allowing them to cool, what
would you expect to happen?
Would appreciable amounts of bacterial DNA be found associated in a helix with
mammalian DNA?

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