MAKE THEM AS BRIEF AS POSSIBLE. (THE ANSWERS) : Lecture 33 Heterologous protein

Question

MAKE THEM AS BRIEF AS POSSIBLE. (THE ANSWERS) :

Lecture 33 Heterologous protein production 1) 2) 3) 4) Disadvantages of engineering a eukaryotic protein in an organism that is prokaryotic Aspects of protein translational regulation that are key for protein folding Generalized eukaryotic vector-what's the main idea? Main concepts with yeast expression- not memorizing any details of names of vectors or promoters 5) Yeast culture- advantages and disadvantages 6) Advantages of expressing proteins in mammalian systems (i.e. CHO cell)

Explanation / Answer

1) Problems to th eprokaryotic host -

6) The mammalian expression system has unique advantage in protein initiation signals, processing, secretion, glycosylation and is suitable for expressing intact macromolecules.

The foreign protein produced by mammalian cells, which is closer to the native protein, is more functional than proteins produced by prokaryotic expression system or by other eukaryotic expression system, such as yeast and insect cells.

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