1. The area labeled as 2 will start replication by putting a primer down: a. Rig
ID: 270918 • Letter: 1
Question
1. The area labeled as 2 will start replication by putting a primer down:
a. Right next to the ori
b. Further away from the ori
2. The area labeled as 1 will complete replication using:
a. Leading strand synthesis
b. Lagging stand synthesis
3. The nontemplate strand for replication is the:
a. Top strand
b. Bottom strand
c. Neither strand
4. The overall direction of replication will be in the opposite direction of replication for which areas of the replication bubble? Cicle all that apply
1 2 3 4
5. An mRNA primer requires a 3' OH in order to synthesize a new strand
a. True
b. False
Explanation / Answer
The direction of the DNA strand with area 2 is in 5’ to 3’ direction. DNA replication always takes place in a 5’ to 3’ direction. The area 2 will start replication by putting a primer down further away from the ori site. This is because replication takes place in a discontinuous manner in 5’ to 3’ direction at the area labeled 2. So, the correct option is option B, further away from the ori site. The area labeled as 1 will complete replication using leading strand synthesis. It is because replication in the area 1 takes place continuously in a 5’ to 3’ direction. So, the correct option is option A, leading strand synthesis. In DNA replication both the strands serve as templates for the synthesis of new strands of DNA molecules. There will not be any nontemplate strand for DNA replication. So, the correct option is option C, neither strand. The overall direction of replication will be in opposite direction of replication for the areas 2 and 3 of the replication bubble. As DNA synthesis takes place in 5’ to 3’ direction, the areas 2 and 3 will be synthesized discontinuously and in a direction opposite to the direction of unwinding of DNA. DNA polymerase cannot synthesize new strands of DNA without a primer. They add deoxyribonucleotides to the 3’-OH group of the primer. DNA polymerase requires a 3’ – OH in order to synthesize a new strand of DNA. The statement that an mRNA primer requires a 3’-OH in order to synthesize a new strand is FALSE.
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