You have a double-stranded DNA the sequence of which is “ATGGTTCTAGGTAAACCTCAA.”

Question

You have a double-stranded DNA the sequence of which is “ATGGTTCTAGGTAAACCTCAA.” Now, you are doing DNA polymerase reaction using: (i) DNA polymerase (ii) dATP, dGTP, dCTP and dTTP+ ddATP 32 (1/10 of dATP) (iii) primer: ATGGTTCTAGGT (underlined)

(Questions 64 & 65) You have a double-stranded DNA the sequence of which is "ATGGTTCTAGGTAAACCTCAA." Now, you are doing DNA polymerase reaction using: (i) DNA polymerase (ii) dATP, dGTP, dCTP and dTTP+ ddATP32 (1 /10 of dATP) (ii) primer: ATGGTTCTAGGT (underlined) 64. How many types of DNA fragments are you making from this reaction? A) 1 B) 4 C) 5 D) 13 E) 14 65. How many types of DNA fragments labeled with P32 (therefore visible) are you making from this reaction? A) 1 B) 4 C) 5 D) 13 E) 14

Explanation / Answer

after primer binding, there are 5 A is present. so the ddATP will incorporate in 5 places of this DNA. so 5 fragments will be produced. option C.

since ddATP will incorporate in 5 places so 5 types of DNA fragments labeled with P32. option C.

ATGGTTCTAGGTAAACCTCAA.

ATGGTTCTAGGTddA

ATGGTTCTAGGTAddA

ATGGTTCTAGGTAAddA

ATGGTTCTAGGTAAACCTCddA

ATGGTTCTAGGTAAACCTCAddA

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