02. You performed tissue printing in the laboratory uwing a munber of plans tiss
ID: 270365 • Letter: 0
Question
02. You performed tissue printing in the laboratory uwing a munber of plans tisse. The per enzyme was observed in ali the plants studied, however the expression level was nok the same in all t plants. Some plants expressed a much higher level of peroxide then others? Please explain how the expression level of peroxidase will relate to the physiology of the plane? Q3. ELISA test is routinely done to test for AIDS virus infection. By using a simple flow diagram to explain the procedure you'll undertake to take to test for the presence of AIDS virus in a homan blood sample?Explanation / Answer
The peroxidase is the enzyme which can use Hydrogen peroxide to catalyse the different reaction. In such process, Hydrogen peroxide is degraded thereby reducing the hydrogen peroxide content in the cell. The Hydrogen peroxide is the reactive oxygen species which can damage the cell if not taken care of properly.
The level of peroxidase enzyme varies with the tissue type and type of stress the plant is experiencing. The levels of the enzyme will be higher in the stroma, chloroplast, mitochondria, thylakoids..etc. It is also elevated in response towards wounding. The higher the metabolism or reaction occurring inside the cell, higher will be hydrogen peroxide levels, we find more peroxidase enzymes in such cases.
ELISA is a very powerful technique to identify the specific antigens or antibodies in the blood. If we want to identify the HIV which causes AIDS ELISA is the easiest method available.
we will be detecting the antibodies against the HIV virus in the blood. We need to have HIV antigens coated on an ELISA plate.
1. Collect the blood form HIV patient and centrifuged to obtain the upper clear liquid (Serum), which contains the HIV antigens.
2. flood the wells with the blood serum of the patient
3. Wash after some time
4. Add Biotinylated antibodies.
5. Washing.
6. Incubation with avidin-biotin-peroxidase complex
7. Wash.
8.Signal detection for confirmation of binding.
The signal indicates the presence of antibodies against the HIV antigen. This confirms the presence of HIV antibodies in the patient. Hence the patient might have HIV infection.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.