201 roose the one best answer 23. Landa ONA could b NOT used for A Recombinant D

Question

201 roose the one best answer 23. Landa ONA could b NOT used for A Recombinant DNA lechology C Constructing DNA lbraries 25 Com out at . spede naence mmune system of bactena aganst war DNA restiction enaymes D. Recognine 4-8-or & base pair, palindromic sequances ?. E. AR 26. A DNA vector and genomie DNA are both out with the same resiniction enzyme. The genomic ONA is shaded. Which of same restriction hesee diagrams depicts a hybrid vector, which can be used to clone the ONA? A. C. D. 27. Restriction enzymes: A Make copy of DNA B. Neaded for ranscripton C.Cut DNA at specitic nucleotide sequence D. A A Is made from stem cells C. Are naturally occurring B. Contains a segment of DNIA from a foreign source D. Uses DNA probe 28. Recombinant DNA (DNA 23. DNA fragments are separated through gel electrophoresis using A. Southen blot B. Northem blot C. Westerm blt 30. Steps of PCR includes A Denaturation, 94-C B. Annealing of primers, 55 C C. Taq Polymerase action, 72-C D. A 31. PCR has been used to: A. Complete Human Genome Projedt B. To identify criminals C. Both A & B 32 For insertion of small amounts of DNA into bacteria we use: A Plasmids B. Virus C. Yeast artficial chromosomes D. A 33. Plasmids are: A Bacterial chromosome with more than 1000 genes B Small extrachromosomal circular DNIA with only few genes C. Restriction enzymes 34. In biotechnology, reverse transcriptase enzyme is used to make: 35. Gene splicing removes 36 Pure preparations ofDNA and RNA haveODN0Dao vales of.A0-1 D. Segment of human chromosome A. cDNA C. Genes B. 18-20 B.RNA C. DNA A. Exon B. Introns D. Plasmids ?.20-28 D. 50 37. In DNA the areas that do not code for amino acids are known as: A. Genome B. Exons C.Introns D.Phenomes 38. One gene may produce: A. Only 1 protein B.Only 2 proteins C. More than 1 protein D. Protein plus fat 39 A DNA polymerase lll B. RNA polymerase C. Restriction endonuclease D. Reverse transcriptase E.Dehydrogenase 40. Electrophoresis apparatus consists of A. Power pack &electrophoresis; unit B.DNA separator&electrophoresis; unit C. Bufferchamber & electrophoresis unit D. Gel, buffer chamber & power pack P.2/2

Explanation / Answer

Lambada DNA could be cut by the ALL restriction endonuclease

Restriction endonuclease is not used for Transcription

Restriction enzyme All

The diagram C depict the hybrid vector which can be used to clone the DNA

Restriction enzyme cut the DNA at specific nucleotide sequence

Recombinant DNA (rDNA) contains a DNA from foreign source

DNA fragments are separated through gel electrophoresis using

Southern Blot

Steps of PCR include

Denaturation at 94 0C, Annealing of Primers at 55 0C, Taq polymerase action at 720C

PCR has been used to Complete Human genome Project, to identify criminals

for insertion of small amounts of DNA into bacteria we use Plasmid

Plasmids are small extrachromosomal chromosome circular DNA with very few genes

In biotechnology reverse transcriptase enzyme is used to make cDNA

gene splicing removes Introns

Pure preparation of DNA and RNA have OD 260/OD280 values of 1.8 – 2.0

In DNA the areas that do not code for amino acid known as Intron

The gene may produce more than 1 protein

The enzyme that is found in the retroviruses and that is required for the synthesis of DNA from RNA is Reverse Transcriptase

electrophoresis apparatus consist of Power pack and electrophoresis Unit

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