6) How does a genomic library differ from a cDNA library? A) A genomic library c

Question

6) How does a genomic library differ from a cDNA library?

A) A genomic library contains coding and noncoding sequences, whereas a cDNA library contains only coding sequences.

B) A genomic library varies, dependent on the cell type used to make it, whereas the content of a cDNA library does not.

C) A genomic library can be made using a restriction enzyme only, whereas a cDNA library requires plasmids and DNA ligase.

D) The genomic library can be replicated but not transcribed.

E) The genomic library contains only the genes that can be expressed in the cell.

Explanation / Answer

A genomic library represents all the genes and is extracted from genomic DNA. It contains both coding and noncoding sequences such as promoters and introns. It is same for all cells in an organism .

A cDNA library is obtained by reverse transcription of mRNA. It contains only coding sequences/ exon. Promoters, introns and other regulatory sequence are absent in it. It does not represent all the genes and also, it differs for different cells because not all cells express same proteins or at equal concentration.

The correct choice is A).

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