3) (from a previous final) A partial diploid was generated by transforming E. co

Question

3) (from a previous final) A partial diploid was generated by transforming E. coli with a plasmid containing lacl, the promoter and operator for the lac operon, and the lac operon. The genotypes of the partial diploid mutants with respect to the lac operon are shown below. +, indicates wild-type, -, indicates mutant, c represents constitutive, s represents no binding to inducer, i represents lacl, p represents the lac operon promoter, o represents the lac operon operator, z represents lacZ, y represents lacY, and a represents laca genotype Which strain(s) will show the listed phenotypes? If no strain will show the phenotype, please indicate as "none" I. constitutive production of o-galatosidase, but normal production of permease and transacetylase. II. super-repression of the lac operon III. constitutive expression of the three genes within the lac operon

Explanation / Answer

1. = Strain 3 , constitutive for beta galactosidase and normal for lac Y and lac A

2. None

3. = Strain 1, constitutive for lac operon Z, Y & A

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