15. What do DNA polymerases require for replication to begin? A) deoxynucleoside

Question

15. What do DNA polymerases require for replication to begin? A) deoxynucleoside 5'-triphosphates B) single-stranded template DNA C) a primer strand to elongate D) All of the above. E) None of the above. 17. What function does helicase perform during replication? A) It stabilizes the negative charge of the transition state B) It utilizes ATP to power strand separation. C) It adds negative supercoils to DNA D) A and C E) None of the above. 19. RNA polymerase requires which of the following for initiation? A) DNA primer B) RNA primer C) supercoiled DNA D) nicked DNA E) None of the above. 21. (2 pts) Eukaryotic mRNA is: A) linear. B) circular C) modified only at the ribose. D) discontinuously translated E) None of the above. 23. (2 pts) Which of the following E. coli proteins is responsible for translocation of the mRNA as a result of a conformation change due to the hydrolysis of GTP? A) RRF B) EF-Tu C) EF-G D) Shine-Delgarno E) None of the above.

Explanation / Answer

15. The correct answer is C) a primer strand to elongate.

17. The correct answer is B) It utilizes ATP to power strand separation.

19. The correct answer is E) None of the above. RNA polymerase doesn't require primer for initiation.

21. The correct answer is B) Circular. Eukaryotic mRNA molecules undergo circularization for efficient translation.

23. The correct answer is C) EF-G. EF-G binds to GTP and undergoes conformational change due to GTP hydrolysis.

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