14. You plan to use the polymerase chain reaction to amplify part of the DNA seq

Question

14. You plan to use the polymerase chain reaction to amplify part of the DNA sequence shown below, using oligonucleotide primers that are hexamers matching the regions shown in red. (In practice, hexamers are too short for most purposes). State the sequence of the primer oligonucleotides that should be used, including their polarity (503), and give the sequence of the DNA molecule that results from amplification. 5'-TAGGCATGCAATGGTAATTTTTCAGGAACCAGGGCCCTTAAGCCGTAGGCAT-3' 3'-ATCGGTACGTTACCATTAAAAACTCCTTGGTCCCGGGAATTCGGCATCGGTA-S

Explanation / Answer

Forward primer: 5'-GCAATG-3'
Length = 6 ntd
GC content = 50%
Tm = 18'C

Reverse primer: 5'-CGGCTT-3'
Length = 6 ntd
GC content = 67%
Tm = 20'C

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