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1) Pseudomonas aeruginosa and Enterococcus faecalis usually grow harmlessly in t

ID: 269111 • Letter: 1

Question

1) Pseudomonas aeruginosa and Enterococcus faecalis usually grow harmlessly in the body. However these organisms can cause disease. Because these bacteria use the Entner- Doudoroff pathway instead of glycolysis to catabolize glucose, a clinical test can be used to provide evidence that the Entner-Doudoroff pathway is functioning and therefore confirm the presence of these potential pathogens. ubstrate A) Assume you have the ability to detect any organic molecule. Name a s molecule you would expect to find in Pseudonionas and Enterococcus cells, but not in human cells. Why? B) In class we determined the number of ATP, GTP, NADH, and FADH, that are formed upon the conversion of glucose to carbon dioxide via the reactions of standard glycolysis (the Embden Myerhoff pathway) and the citric acid cycle. How many of each of these molecules are formed if glucose is converted to carbon dioxide using the Entner-Doudoroff pathway and the citric acid cycle instead?

Explanation / Answer

1) the molecule is gluconolactone absent in human but present in Pseudomonas and Enterococcus. they do not have enzyme phosphofructokinase which is present in human. so they are using Entner-Doudoroff pathway.

2) during glycolysis we got 2 ATP and 2NADH

during Entner-Doudoroff pathway we got 1ATP, 1NADH and 1 NADPH molecule.

rest citric acid will produce the same amount of ATP, GTP, NADH and FADH2 molecules.