Ro-3306 inhibits one of the Cdks in the cell cycle control system. A cervical ca
ID: 268945 • Letter: R
Question
Ro-3306 inhibits one of the Cdks in the cell cycle control system. A cervical cancer cell line called HeLa was treated with Ro-3306 for 30 hours. During the treatment, cells were sampled and went through flow cytometry, from which their DNA content were measured. The flow cytometry plots for each time point are shown in the figure below. Answer the following questions based on the plots. (10 pts) Incubation time with Ro-3306 0 hr 24 hr 30 hr 0 200 400 6000 200 400 600 0 200 400 600 DNA contents 11a) Based on the data, how does Ro-3306 impact cell cycle progress in Hela cells ? if you think cell cycle is arrested, point out which phase the cells are arrested at, (5 pts) 11b) You suspect that Ro-3306 is affecting either S-Cdk or M-Cdk. Based on the data above which Cdk is less likely to be affected by Ro-3306? Explain your answer (5 pts)Explanation / Answer
Answer 11a:- Based on the data of the flow cytometry assay it can be easily seen that the compound Ro-336 arrests the cell cycle in S and G2/M phase. As can be seen the peak for G1 phase is decreasing in a time dependent manner from 0 hour to 30 hour, and the peaks for S phase and G2/M phases is increasing. So it can be said that this compound arrests the cells at S and G2/M phase.
Answer 11b:- As the cells are being arrested in S and G2/M phases therefore the CDKs of G1 phase will not be affected by this compound. The CDKs for G1 phases are CDK4, CDK6 and CDK2, although CDK2 is also involved in S phase so there may be some effect on its activity.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.