BIOL 2035 Spring 2018 [L. Thrun, PhD) NAME Lab Quiz #4 USE THE BLUE SIDE 8. The
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BIOL 2035 Spring 2018 [L. Thrun, PhD) NAME Lab Quiz #4 USE THE BLUE SIDE 8. The urea hydrolysis test can. c. determine your risk for dental car d. differentiate Gram positive cocci a. detect bacteria that can colonize the urinary t b. differentiate members of Enterobacterioceae. SIM 9. The SIM medium is a combination medium. That means. e different kinds of bacteria can be detected in this test. b, mixed cultures are used to inoculate this medium c. more than one result will be obtained from one inoculation in this one medium. d. more than one n e. all these apply to the term combination medium 10. How would Turbidity throughout the tube and a pink color after adding the Kovac's you interpret the following: reagent. Mark all that apply d. The bacterium produces acid e. The bacterium reduces sulfur a. The bacterium is motile. b. The bacterium degrades tryptophan c. This is a negative result. Gel electrophoresis 11. DNA runs to the pole because DNA is charged a. positive pole // positively b. negative pole// positively d. negative pole// negatively c positive pole / negatively 12. a. Larger I/ slowly b. Larger// quickly Blood Agar Plate pieces of DNA move more through the gel c. Smaller I/ slowly d. Shorter / slowly 13. You inoculate a BAP with a swab from your throat. You observe a yellow halo or clearing around tiny, white colonies. What conclusions can you make? a. This i b. Thi s an example of alpha hemolysis indicating the microbe makes a weak hemolysin. s is an example of gamma hemolysis indicating the microbe makes a strong hemolysin C. This is an example of beta hemolysis indicating the microbe makes a weak hemolysin. d. This is an example of gamma hemolysis indicating the microbe makes a weak hemolysin e. This is an example of beta hemolysis indicating the microbe makes a strong h 14. We've inoculated media containing blood twice this semes ethyl alcohol. As a result... ter. The second time, the medium did not contain phenyl a. These plates are not differential d. This test only allows Gram positive bacteria to grow. b. This test is differential only. e. This test is selective only c. There is no difference with the type of results one would get with or without PEA. Enterotube 15. You suspect your patient has an infection caused by a member of Enterobacteriaceae. After inoculating an enterotube, you quickly realize this is not the case. Which of the following results would lead you to that conclusion? . Negative for gas production. . Negative for indole degradation d. Negative for glucose fermentation e. Positive for indole degradation. Positive for glucose fermentation.Explanation / Answer
8. Ans. a) Detect bacteria that can colonize the urinary tract - Because these bacteria are secreting the Urease enzymes which can be detected by Urea hydrolysis test.
9. Ans. c) More than one result will be obtained from one inoculation in this medium - Because SIM medium tests three different parameters (represented by the three letters in the name: Sulfur Reduction. Indole Production and Motility).
10. Ans. b) the bacterium degrades Tryptophan - Tryptophan is an amino acid that can be oxidized by some bacteria and converted to indole, pyruvic acid, and ammonia. Detection of indole indicates tryptophan degradation. The active ingredient in Kovacs Reagent, p-dimethylaminobenzaldehyde, reacts with indole to form a pinkish-red end product that is highly visible.
11. Ans. c) Positively poles // negatively charged - the DNA contains net negative charge due to presence of Phosphate (containing O-ve) molecules in their backbone. Which results in the movement of DNA towards positively poles in gel electrophoresis.
13. Ans. e) This is an example of beta hemolysis indicating the microbe makes a strong hemolysis - Beta hemolysis is also called as complete hemolysis, it results in complete lysis of red cells in the media around and under the colonies and the area appears lightened yellow or transparent.
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