1. Urokinase-type Plasminogen Activator (uPa) is a serine endopeptidase. The act

Question

1. Urokinase-type Plasminogen Activator (uPa) is a serine endopeptidase. The active site of this peptidase binds the peptide-motif Ser-Gly-Arg-Ser-Ala and cleaves the protein/peptide between arginine and serine. What is the sequence of the products of the oligopeptide Ala-Gly-Ser-Gly-Arg-Ser-Ala-Lys-Gly-Ala-Ser-Asp after cleavage by uPa? a) Ala-Gly and Ser-Gly-Arg-Ser-Ala-Lys-Gly-Ala-Ser-Asp b) Ala-Gly-Ser-Gly-Arg and Ser-Ala-Lys-Gly-Ala-Ser-Asp c) Ala-Gly-Ser-Gly-Arg-Ser-Ala-Lys-Gly-Ala-Ser and Asp d) Ala-Gly-Ser-Gly-Arg-Ser and Ala-Lys-Gly-Ala-Ser-Asp 2. The tertiary structure of proipinsdepends primarily on which property of amino acids? I) disulfide bonds II) hydrogen bonds III) amide bonds IV) polar side chains a) III, IV b) I, IV c) II, III Which physical property of triacylglycerols rich in saturated fatty acids is responsible for the higher melting point than the corresponding unsaturated compounds? a) Dispersion forcès b) Hydrophilic interactions c) Cis-carbon-carbon double bond steric interactions d) Hydrogen bonding 3. Which reagents could be used to harden an oil to a fat? a) NaBH,/HO b) Ni /H 4. d) Cu/buffer

Explanation / Answer

1) Ala- gly-ser-gly-arg-ser-ala-lys-gly-ala-ser-asp

uPa cut between arg-ser residue

so the sequence after cleavage by uPa is

option B. Ala- gly-ser-gly-arg and ser-ala-lys-gly-ala-ser-asp

2) option A. disulfide bonds and polar side chain.

3) option B. hydrophobic interactions.

4) option B. Ni/H2 . this process is called hydrogenation of oils.

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