inlets for water sheaths and one straight channel. 3) The third stage has one ce
ID: 267954 • Letter: I
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inlets for water sheaths and one straight channel. 3) The third stage has one center inlet for DPPC, DSPE-PEG, and cholesterol in ethanol and a spiral channel (Scheme 1 and Figure S1 in the Supporting Information). In this work, the flow rate ratio (FR) of side inlets to middle inlet at different stages is optimized for synthesis of RNV, and the production rate of RNV is 114 mgmin¢1 by the microfluidic chip (Supporting Information). The transmission electron microscopy (TEM) image of an assembled RNV collected from the outlet shows a hollow core–shell structure with a bright water core and an intact PLGA shell (Figure 1A). We use the positively charged DOTAP to form the reverse micelle as well as the inner surface of PLGA shell because DOTAP can interact with the negatively charged siRNA, thus ensuring an efficient encapsulation of siRNA inside the water core. Meanwhile, the lipids (DPPC, DSPE-PEG, and cholesterol) are assembled onto the outer surface of PLGA shell in the third stage of microfluidic chip, in order to achieve a longterm stabilization and a long circulation time. We first encapsulate hydrophilic reagents, such a
tell me why this happen in easier way and what is the principle ??what is PLGA use here????
and why reverse micelle formed??? and PLGA shell is oil phase???
DPPC&DSPE-PEG; PLGA&DOTAP; Water &Cholesterol; in Ethnol in DMF Hydrophilic reagents in Water Lipid layer TAR PLGA shell Water core (Reverse micelle) Scheme 1. Schematic of the water core/PLGA shell/lipid layer rigid nanovesicle (RNV) assembled by the three-stage microfluidic chip in one-stepExplanation / Answer
PLGA - poly lactic co glycolic acid is used here because it can be easily taken up by the cells. If it is coupled with cancer drug can be effective in the treatment of cancer. Moreover , when PLGA is coupled with hydrophobic interior it is taken up by the cells easily compared to PLGA - lipid combination. When the drugs, nucleic acids and other hydrophilic reagents in treatment of anti cancer when injected have lifetime only for a short period. That is why the drugs are coupled with PLGA given in cancer treatment. The PLGA has outer lipid core which makes increased circulation time. Reverse micelles are formed because the outer region of the micelles which interacts with water when present in hydropilic surface and also at the same time contains hydrophobic drug inside it. This is the condition of the human body and micelles should stay in hydrophilic environment and should excert action . That is why reverse micelles are formed. Inside the PLGA shell it contains hydrophobic drugs and hence these drugs cannot interact with hydrophilic particles and hence the PLGA is in oil phase.
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