1. An example of a mutation that can often create a cis -dominant acting non-ind
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Question
1. An example of a mutation that can often create a cis-dominant acting non-inducible variant of the E. coli lac operon would be:
A. a nonsense mutation in the lacZ gene.
B. a deletion of the operator region.
C. a deletion mutation within the promoter.
D. a nonsense mutation in the CAP gene.
E. a mutation leading to high level expression of the lacI gene
2. In the context of cloning vectors, a large number of restriction enzyme recognition sequences clustered into a short segment of DNA would be called a/an:
A. ori.
B. Polylinker (or MCS).
C. ARS.
D. RES.
E. VRE.
3. n the context of eukaryotic genetic material, the 30 nm fiber is:
A. the DNA double helix.
B. a string of nucleosomes.
C. a coil of nucleosomes with about 6 per turn.
D. a repeatedly folded fiber of chromatin.
E. the chromosome scaffold.
4.The process of gene conversion is most closely associated with:
A. DNA replication.
B. mismatch repair.
C.mutations associated with mitochondria.
D.recombinant DNA techniques.
E. chromatin remodeling.
5.The “additional” activity (beyond polymerization) that is specifically required for a DNA polymerase to carry out the process of nick translation is:
A.a helicase activity.
B.3’exonuclease activity.
C. 5’exonuclease activity.
D.a 5’endonuclease activity.
E.a 3’topoisomerase activity.
6. The VNTR minisatellite sequences/loci have been suggested to be involved in the process of:
A.initiating DNA replication.
B.regulating transcription.
C.homologous recombination.
D.kinetochore formation.
E.replicating linear chromosome ends.
7.An example of an enzyme that consists of both protein and RNA is:
A. DNA polymerase I.
B. gyrase.
C. telomerase.
D. DNA ligase.
E. reverse transcriptase.
8. During the process of nick translation (as part of the process of DNA replication), the enzyme is using:
its helicase activity.
A. its 5’exonuclease activity.
B. its 3’exonuclease activity.
C. its primase activity.
D. All of the above.
9. The origins of DNA replication in yeast are called:
A. SINES.
B. VNTRs.
C. LINES.
D. Alu elements.
E. ARS.
10. The enzyme responsible for carrying out the process of nick translation on the lagging strand during DNA replication in E. coli is:
A. helicase.
B. primase.
C. DNA polymerase I.
D. DNA polymerase III.
E. gyrase.
Explanation / Answer
1) option C. a deletion mutation within the promoter region.
2) option B. polylinker or Multiple cloning site.
3) option C. a coil of nucleosomes with about 6 per turn.
4) option A. DNA replication.
5) option C. 5' exonuclease activity of Pol I helps in nick translation process.
6) option C. telomerase protein.
7) option A. 5' exonuclease activity.
10) option C. DNA Pol I.
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