How would you devise a screen to identify recessive mutations in Drosophila that

Question

How would you devise a screen to identify recessive mutations in Drosophila that result in embryo lethality? How would you propagate the recessive mutant alleles?

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heterozygous homozygous hemizygous dark light curly straight for dark body (db) are fed EMS to induce mutations and then mated to Male flies females marker, CyO. Individual F, males that have selected and mated back to a female from the chromosome 3 balancer strain to create man independent Fa lines. Fa progeny with heterozygous for the new mutations. Interbreeding the F2 will create many independent Fs populations. If the F2 were winged flies among the F3. The curly-winged Fo will be used to generate fly stocks carrying the new mutant allele for a balancer chromosome 3 containing the db allele and the dominant wings and bodies are wings and bodies will be for a recessive embryonic lethal mutation, there would be no straight for the mutations and can be

Explanation / Answer

Male flies homozygous for dark body (db) are fed EMS to induce mutations and then mated to females heterozygous for a balancer chromosome 3 containing the db allele and the dominant marker, CyO. Individual F1 males that have curly wings and dark bodies are selected and mated back to a female from the chromosome 3 balancer strain to create many independent F2 lines. F2 progeny with curly wings and dark bodies will be heterozygous for the new mutations. Interbreeding the F2 will create many independent F3 populations. If the F2 were heterozygous for a recessive embryonic lethal mutation, there would be no straight-winged flies among the F3. The curly-winged F3 will be heterozygous for the mutations and can be used to generate fly stocks carrying the new mutant allele.

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