Which of the following statements concerning a genomic library is true? It can b
ID: 266771 • Letter: W
Question
Which of the following statements concerning a genomic library is true? It can be made only from cDNA. It comprises the genomic DNA from an organism. It can be made only for eukaryotic organisms. Every colony in the library has only one gene. Reverse transcriptase is essential to construct the library. cDNA libraries A. are likely to contain all protein-coding genes. B. require DNA polymerase for their construction. C. include DNA from noncoding sequences. D. are the same as genomic libraries. E. require reverse transcriptase for their construction. Which of the following statements concerning a genomic library is true? It can be made only from cDNA. It comprises the genomic DNA from an organism. It can be made only for eukaryotic organisms. Every colony in the library has only one gene. Reverse transcriptase is essential to construct the library.Explanation / Answer
statements concerning a genomic library:
Ans: It comprises the genomic DNA from an organism
Construction of a genomic DNA library involves isolation, purification and fragmentation of genomic DNA followed by cloning of the fragmented DNA using suitable vectors.
cDNA libraries:
Answer: require reverse transcriptase for their construction.
mRNA being single-stranded cannot be cloned. It is first converted into DNA before insertion into a suitable vector which can be achieved using reverse transcriptase (RNA-dependent DNA polymerase or RTase) obtained from avian myeloblastosis virus (AMV).
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