enzyme A enzyme B Figure 3: Metabolic Pathway Refer to the metabolic pathway ill
ID: 266701 • Letter: E
Question
enzyme A enzyme B Figure 3: Metabolic Pathway Refer to the metabolic pathway illustrated in Figure 2. Substance C is required for the cell's growth. In your experiment, you irradiate the cell's DNA to cause mutations to the enzyme genes. Sure enough, the cell doesn't grow after radiation unless supplemented. Now you need to figure out which gene was mutated. Use this information to answer the questions belovw. 5. You supplement your mutated cell culture with substances A, B and C. Do the cells grow? Why or why not? You supplement your mutated cell culture with substance B only. The cells grow! Is enzyme B's gene mutated? How do you know? 6. 7. You supplement your mutated cell culture with substance A only. The cells do not grow! Is enzyme A's gene mutated? How do you know?Explanation / Answer
Please find the answers below:
Answer 5: Yes, the cells will grow. The information clearly suggests that the substance C is essentially required for growth of the cells. Hence, supplementation with all A, B and C will promote cell growth independent of the fact that either or all of them are mutated.
Answer 6: Since bio-conversion of B to C takes place using an enzyme and the mutated cell is able to transform B to C, it suggests that the enzyme required for this bio-conversion remains intact in the cells.
Answer 7: Since sequential steps are required for bio-conversion of A to B and then C, failure of cells to grow on a medium containing only A suggests that the enzyme required to convert A to B is mutated and suffered from loss-of-function. This prevented cell growth.
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