A Drosophila transgene has coding sequences for GFP positioned downstream of a 7

Question

A Drosophila transgene has coding sequences for GFP positioned downstream of a 7-kb DNA fragment taken from the segment-polarity gene wingless (wg). In Drosophila embryos, the transgene expresses GFP fluorescence in exactly the same pattern as Wg. Crosses are used to place the transgene in different mutant backgrounds.

a. Describe, with words and diagrams, the pattern of GFP fluorescence you expect to see in each of the following types of embryos if they are examined at germ-band extension.

i. wild-type

ii. embryos laid by a mother who lacks the function of the maternal-effect gene bicoid

iii. embryos that lack the function of the pair-rule gene even-skipped

iv. embryos that lack the function of the segment-polarity gene engrailed

v. embryos having a mutation in the Hox gene Ultrabithorax that leads to a homeotic transformation of T3, the third thoracic segment which normally has a haltere, to T2, the second thoracic segment which normally has a wing.

Explanation / Answer

i)

ii)

iii)

iv)

v)

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