124) PCR uses three steps repeatedly to make copies of DNA. This step joins prim

Question

124) PCR uses three steps repeatedly to make copies of DNA. This step joins primers to their target sequence on the template strand A) Denaturation, B) Annealing C) Extension D) Initiation E) Termination 125) The reagents used in PCR include all of these except 126) This nucleotide is used to terminate DNA synthesis during the DNA sequencing? 127) This technique is used to identify a specific RNA within a mixture RNA molecules A) dNTPs B) primers C) Tag DNA polymerase D) DNA ligase E) DNA template A dTTP B) dATP C) dCTP D) dGTP E) ddATP A) RT-PCR D) Gel retardation assay B) Southern blot E) DNA footprinting C) Western blot 128) You expect to see this restriction site GTNAYC every DNA substrate has a random sequence with equal amounts of each of the bases. The symbolN stands for any nucleotide, R for any purine (A or G), and Y for any pyrimidine (T or C base pairs assuming that the A) 256 B) 512 C) 1024 D) 6 E) none of the abov 129) is used to synthesize cDNA from mRNA before a cDNA library is made. A) DNA polymeraseB) DNA ligase D) Reverse transcriptase E) helicase C) RNA polymerase

Explanation / Answer

In annealing step only the primers join the target sequence on the template strand.
option b is the correct answer

DNA ligase is not used in PCR.
Option d is the answer

In sequencing, di-deoxynucleotidetriphosphates are used to terminate DNA elongation.
Hence answer is ddATPs.
Option e is the answer

RT-PCR is used to identify to identify specific RNA within a mixture of RNA molecules.
Option a is the answer

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