PLEAS PROVIDE AN EXPLANATION FOR THE ANSWER CHOICES. Your favorite gene is the L
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PLEAS PROVIDE AN EXPLANATION FOR THE ANSWER CHOICES.
Your favorite gene is the Lemon gene and you would like to determine whether the Lemon gene is found in tightly or loosely compacted chromatin. Which of the experimental set-ups would allow you to accomplish this? Circle all that apply. A. PCR using Lemon specific primers to see if a product is made. B. Digest Lemon containing chromatin, and see if DNA remains to hybridize with a Lemon probe. C. Stick aLemon plasmid into an vitro transcription assay and determine whether Lemon mRNA is produced.Explanation / Answer
When we do PCR using specific primers of Lemon then we will get the PCR product. But this PCR will not tell us about the level of gene expression of Lemon. So option A is wrong
When we digest the Lemon containing chromatin and then hybridize the DNA with a probe, it will tell the presence or absence of Lemon gene in the chromatin. So this option is also wrong
In in-vitro transcription assay, we can get to know the expression level of the gene of our interest. In-vitro transcription assay expression level of any gene can be determined by using the DNA of the gene. Amount of mRNA tell us the level of transcription of the gene
So correct answer is option C.
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