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Most organisms display a circadian rhythm, in which biological processes are syn

ID: 264619 • Letter: M

Question

Most organisms display a circadian rhythm, in which biological processes are synchronized with day length (e.g., in humans, rapid movement between time zones results in jet lag, in which established circadian rhythms are out of sync with daylight hours). In Drosophila, pupae eclose (emerge as adults after metamorphosis) at dawn Part A Using this knowledge, how would you screen for Drosophila mutants that have an impaired circadian rhythm? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Terms can be used once, more than once, or not at all. Reset Help dawn Using eclosion at times other than dawn as the mutant phenotype, i is possible to conduct an F3 screen for recessive mutations that affect the time pupae eclose. Male flies heterozygous for dark body (db) are fed EMS to induce mutations and then mated to females heterozygous for a balancer chromosome 3 containing the db allele and the dominant marker, CyO, which causes curly wings Individual F1 males that have mated back to a female from the chromosome 3 balancer strain to create many indepennt F2 lines F2 progeny with mutations. Interbreeding the F2 will create many independent F3 populations. If the F2 were heterozygous for a circadian rhythm mutation, about one-third of the progeny would eclose at dusk daytime times other than dawn wings and bodies are selected and homoz ygous heterozygous hemizygous curly straight wings and bodies will be heterozygous for the new dark light

Explanation / Answer

Most organisms display a circadian rhythm, in which biological processes are syn

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