A Drosophila transgene has coding sequences for GFP positioned downstream of a 7

Question

A Drosophila transgene has coding sequences for GFP positioned downstream of a 7-kb DNA fragment taken from the segment-polarity gene wingless (wg). In Drosophila embryos, the transgene expresses GFP fluorescence in exactly the same pattern as Wg. Crosses are used to place the transgene in different mutant backgrounds.

How would you experimentally identify the protein factors that interact with the sequences required for the normal expression of wg?

i. What would you expect to find, based on work with the even-skipped gene?

Explanation / Answer

A Drosophila transgene has coding sequences for GFP positioned downstream of a 7

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