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In microbiology, dilutions are critical for getting a countable number of microb

ID: 264027 • Letter: I

Question

In microbiology, dilutions are critical for getting a countable number of microbes to grow on a petri dish. If you suspect your culture of bacteria has 210 x 106 cells per mL, what would you want the final dilution to be in order to end up with 30 colonies of bacteria on a petri dish? Express your answer as an exponent rounded to two decimal places. Eg 10.01e-3, not 0.00101 Treat colonies of bacteria (cells /mL) the way you would treat concentration (Moles / L) The formula M1V1 M2V2 can still be used. The final concentration is the number of colonies on the petri dish. Solve for V2

Explanation / Answer

In microbiology, dilutions are critical for getting a countable number of microb

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